Micropropagation micropropagation is the production of whole plants from small sections of plant such as a shoot tip, node, meristem, embryo, or even a seed plant tissue culture is basically the same thing, except that it implies the use of callus tissue generated from plant cells cultured invitro. A complete protocol is presented for the first time for the micropropagation of pongamia pinnata, a biofuel tree, using cotyledonary nodes derived from axenic seedlings. Murashige and skoogs ms basal medium with benzylaminopurine ba, kinetin kn, zeatin z, and thidiazuron tdz were tested for induction of multiple shoots from maturetreederived axillary meristems of pongamia pinnata. Evaluation of antibacterial activity of pongamia pinnata l. It is the truetotype propagation of a genotype that comprises many stepsstock plant care, explant selection and sterilization, media manipulation to obtain proliferation, rooting, acclimation, and growing on of liners and is. Plant tissue culture division, national chemical laboratory. Oct 16, 2007 a complete protocol is presented for the first time for the micropropagation of pongamia pinnata, a biofuel tree, using cotyledonary nodes derived from axenic seedlings.
Research article physico chemical analysis of pongamia pinnata. Enhancing rate of multiplication shoot multiplication short cycle 26 weeks small size propagules storage on large scale, easily transported. By using micropropagation the rate of increase can be speeded up by as much as ten times. Department of biotechnology, gulbarga university, gulbarga585106, karnataka, india corresponding author email. The plant extract contains flavonoids, carbohydrates, glycosides, international journal of chemtech.
Multiple shoots were induced in vitro from nodal segments through forced. The micropropagation of chestnut via axillary shoots involves four stages. A tissue culture technique for plant propagation in which tissue is taken from a plant and grown in a laboratory to produce plantlets that are. A protocol for in wk propagation of this plant was standardized using seedling explants. Micropropagation is an advanced vegetative propagation technology for producing a large number of genetically superior and pathogenfree transplants in a limited time and space. Development of micropropagation protocols would allow commercial tissue culture companies to mass propagate and make available to the nursery trade elite lines of selected ornamental, herbaceous perennials. Read this article to learn about the techniques, factors, applications and disadvantages of micro propagation process and also about production of diseasefree plants and its merits and demerits.
Micropropagation enables growers to increase the production of plants that normally propagate very slowly such as narcissus and other bulbous crops. Pdf micropropagation of karanj pongamia pinnata pierre. Micropropagation of mature pongamia pinnata pierre bioone. Research article physico chemical analysis of pongamia. Micropropagation of ornamental perennials university of. It is often known by the synonym pongamia pinnata as it was moved to the genus millettia only recently. Gavino rotor, 1949 1st in vitro vegetative propagation.
Micropropagation of pongamia pinnata a sustainable biofuel plant species for semi arid climatic conditions vinod kumar patil and naik, g. Unit ii biotechnology and plants lesson 7 plant tissue culture 169 cells and tissue the starting point for micropropagation can be individual cells or small amounts of plant tissue. To 10 g of crude pongamia oil, a known amount of catalyst koh, 0. With orchids it is the only way to reproduce the plants as. Shoot culture 9most widely used method for commercial micropropagation 9relatively high genetic stability in the plants produced shoot culture advantages 9reliable rates and consistency of shoot multiplication 93 8 fold multiplication rate per month 9preexisting meristems are least susceptible to genetic changes micropropagation stages stage 0.
Direct adventitious shoot bud formation on hypocotyls explants in. Micropropagation of mature pongamia pinnata pierre springerlink. Micropropagation of karanj pongamia pinnata pierre through shoot apex segmentsa medicinal and biofuel plant. These non edible oil seeds are also used for lightning purpose at night. Micropropagation in horticulture, by s gunn the plantsman new series vol. Micropropagation is the growing of plants from meristematic tissue or somatic cells of superior plants on nutrient suitable media under controlled aseptic physical conditions. A piece of plant tissue called an explant is a cut from the plant, b disinfested removal of surface contaminants, and c. Its native range is uncertain, with conflicting information in the published literature.
Micropropagation of orchids micropropagation is the process used to replicate plants such as orchids, the word micropropagation means simply propagation in miniature. Sprouting of buds was 64% on medium devoid of plant growth regulators pgr. Department of pharmacognosy 1, small and medium pharmaceutical. Pdf pongamia pinnata pierre is a tree legume, having potential in. Plants can be propagated by sexual through generation of seeds or asexual through multiplication of vegetative parts means. Culture media used in the initiation, shoot multiplication and rooting stages of the micropropagation of european chestnut via axillary shoots. However, the queensland herbarium currently considers. Pdf in vitro regeneration of pongamia pinnata pierre.
Preparation of biodiesel from crude oil of pongamia. Methods of micropropagation micropropagation is the practice of quickly multiplying stock plant material to produce a large number of progeny plants, using contemporary plant tissue culture methods. Micropropagation definition, the propagation of plants from tissue cultures, often from single cells. Chapter2 micropropagation and large scale culture of c. Micropropagation of mature pongamia pinnata pierre. Pongamia trees are legumes so it helps in replenish soil nitrogen. It is done under sterile conditions in a laboratory using plant seed or tissue. It grows about 1520 meters in height with a large canopy which spreads equally wide. Multiplication of genetically identical copies of a cultivar by asexual reproduction using tissue culture techniques iv requirements.
Pongamia pinnata is a fastgrowing, mediumsized, evergreen or briefly deciduous, glabrous shrub or tree with a broad crown of spreading or drooping branches. Murashige and skoogs ms basal medium with benzylaminopurine ba, kinetin kn, zeatin z, and thidiazuron tdz were tested for. Detail study on the properties of pongamia pinnata karanja. Pongamia pinnata is a fastgrowing, mediumsized, evergreen or briefly deciduous, glabrous shrub or tree with a broad crown of spreading or drooping branches 303 title. The labour intensive nature of micropropagation has led to outsourcing of micropropagation from european nurseries to contractors in low wage, typically asian, countries, with the plantlets being returned for growing on. Micropropagation is labour intensive involving manual handling at 3 or 4 stages. Micropropagation of pongamia pinnata through enhanced. One such genus is that of trilliums, and while i do not intend to work on trilliums exclusively, i do intend to concentrate on this group. Useful tropical plants database 2014 by ken fern, web interface by ajna fern with help from richard morris. Pierre leguminaceae, subfamily papilionoideae is a medium sized tree commonly called as karanja that generally attains a height of about 1020 m and a trunk diameter of more than 50 cm pongamia. Pappilonaceae is a nonedible oil of indian origin lakshmikanthan, 1978. The rhs libraries can, for a fee, provide photocopies, by post if necessary. It has been successfully introduced to humid tropic lowland worldwide and to parts of australia, china, newzewland and united states2. Sep 10, 2014 aseptic method of clonal propagation is called as micropropagation and it offer the advantage of large number of truetotype plantlets can be produced with relatively short time and space from a single individual.
Creative commons attributionnoncommercialsharealike 3. General physical properties of pongamia pinnata oil blends b10, b20 and diesel parameters pongamia pinnata diesel b20b10 ph 3. Plant tissue culture offers new ways for the improvement of this crop after many years of recalcitrance. Multiple shoot bud induction and plant regeneration studies of. Plant propagation by tissue culture is known as micropropagation. Vegetative propagation of sterile hybrids can be used as parent plants for seed production.
Learn vocabulary, terms, and more with flashcards, games, and other study tools. Preparation of biodiesel from crude oil of pongamia pinnata. Pongamia pinnata pierre is a tree legume, having potential in production of raw material for biodiesel. Micro propagation is used to multiply dignified plants such as those that have been genetically modified or bred through conventional plant. Article pdf available in journal of pharmaceutical sciences and research 33. Introduction to micropropagation cornell university. Of the various culture media that have been assayed for micropropagation of. Pongamia pinnata is drought resistant, semideciduous, nitrogen fixing leguminous tree. Pierrea native indian biodiesel tree from cotyledonary node article pdf available january 2010 with 740 reads.
Micropropagation is the only viable method of multiplying genetically modified cells or cells after protoplast fusion. Pongamia pinnata grows much faster than any other bush and fixes co 2 as its stem and branches, thus reducing co 2 from atmosphere and offer biomass. Pelagia research library advances in applied science research, 2014, 51. Study the effect of growth regulators on micropropagation of gloriosa superba l. The collection and extraction of oil is carried out by indian biodiesel corporation, baramati. Usually meristem or buds without callus reduced somoclonal variation axillary branching. Application of tissue culture techniques for genetic up gradation of economically important plants has been reported. Aug 18, 2007 murashige and skoogs ms basal medium with benzylaminopurine ba, kinetin kn, zeatin z, and thidiazuron tdz were tested for induction of multiple shoots from maturetreederived axillary meristems of pongamia pinnata.
Pdf murashige and skoogs ms basal medium with benzylaminopurine ba, kinetin kn, zeatin z, and thidiazuron tdz were tested for induction of. Pongamia is a medium size, nitrogen fixing tree native to india, indonesia, malaysia, and myanmar. The main characteristic of this technique is rapid clonal multiplication which results in the production of a large number of uniform plants from a limited number of mother stock plants. Derris indica bennet fabaceae pongam, indian beech. Employing plant tissue culture techniques for micropropagation and further. History of plant tissue culture propagation at cornell. Curcuma longa is an indian spice derived from the rhizomes of the plant and has a long history of use in ayurvedic medicine as a treatment for inflammatory conditions. This is particularly valuable in the case of difficultto. Transesterification of crude oil of pongamia pinnata 2. Millettia pinnata is a species of tree in the pea family, fabaceae, native in tropical and temperate asia including parts of indian subcontinent, china, japan, malaysia, australia and pacific islands. Unit ii biotechnology and plants plant tissue culture 7.
Lewis knudson, 1922 1st in vitro embryo germination orchid ie the invention of in vitro embryo culture. In our country there are more than 300 species of trees, which produce oil bearing trees. Detail study on the properties of pongamia pinnata. Incorporation of ba, kn, or z was ineffective in enhancing sprouting frequency or. Pongamia millettia pinnata, formerly known as pongamia pinnata, is a treeshrub with a broad distribution from india, through central and southeastern asia, indonesia and into northern australia. After tranesterification of crude oil shows excellent properties like calorific value, iodine number, cetane number and acid value etc. Micropropagation propagation in vitro is pres ently the most important commercial application of plant tissue culture. Micropropagation definition of micropropagation by the. The process of micropropagation can be divided into four stages.
Micropropagationomicsgroupjournal of biotechnology and. Pongamia pinnata, floral phenology, pod maturation, fruit colours, seed and seedling quality characters. Micropropagation is the practice of rapidly multiplying stock plant material to produce many progeny plants, using modern plant tissue culture methods micropropagation is used to multiply plants such as those that have been genetically modified or bred through conventional plant breeding methods. Literature survey of pongamia pinnata revealed its potential being like hepatoprotective and antioxidant 30. Pongamia pinnata is regarded as a sustainable biofuel feed. Micropropagation definition of micropropagation by the free. Plant cells for micropropagation may be newly transformed as a result of genetic engineering, or they might be cells taken from plant tissue. Micropropagation offers the potential to produce thousands or even millions of plants per annum.
It is found mainly in the native western ghats in india, northern australia, fiji and in some regions of eastern asia. Micropropagation is a combination of the arts and sciences of plant multiplication in vitroand plant acclimitization. Pongamia pinnata oil with conventional diesel with b10 and b20 proportions are furnished in the table3. Genetic, biochemical, and morphological diversity of the legume.
Pdf micropropagation of mature pongamia pinnata pierre. The micropropagation of chestnut from tissues taken from adult trees appears usually to be feasible when these tissues retain physiologically juvenile characteristics, as is the case of basal shoots and stump sprouts vieitez et al. It is also used to provide a sufficient number of plantlets for planting from a. Lewis knudson, 1922 1st in vitro embryo germination orchid ie the invention of in vitro embryo culture knudson, l, 1922 nonsymbiotic germination of orchid seeds, botanical gazette lxxiii. Principles of tissue cult ure and micropropagation. Recent studies2 4 revealed that most chlorophyllous plants in vitro have the ability to grow photoautotrophically, and that the low co 2 concentration in the airtight. Effects of pongamia pinnata on lipid peroxidation products and antioxidants in hyperammonemic rats with reference to circadian variations were evaluated by essa and subramanian 29. Knudson, l, 1922 nonsymbiotic germination of orchid seeds, botanical gazette lxxiii. Micropropagation micropropagation of mature pongamia pinnata pierre k.
Transesterification of crude pongamia oil using koh. Tissue culture serves as an alternative to vegetative propagation by conventional methods, such as by rooting of cuttings. Multiple shoots were induced in vitro from nodal segments through forced axillary branching. Introduction of disease free new cultivars is possible through micropropagation 6. Pongamia pinnata is a legume plant which has great potential to be used as a. Micropropagation differs from all other conventional propagation methods in that aseptic conditions are essential to achieve success. In case of dioecious species, where one of the sex is more desirable then under such circumstances plants of desired sex can be selectively multiplied by this technique. The database and code is licensed under a creative commons attributionnoncommercialsharealike 3. Micropropagation definition of micropropagation at. Jain 1, shashi alok 1, deepak kailasiya 1, vinod kr. Advantages of micropropagation requires relatively small growing area gives propagules such as minitubersmicrocorms for plant multiplication throughout the year. It is the fact that micropropagation is the only commercially viable method of clonal propagation of most of the horticultural crops. Axillary buds, biodiesel plant, leaf, micropropagation, pongamia, plant growth regulators, plant tissue culture.
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